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SBGridTV

Supported Applications

SPHIRE

  • Description

    (SParx for HIgh Resolution Electron microscopy) for cryoEM using statistical resampling to attain quality assessment and result reproducibility. Includes movie frame alignments (movie), CTF estimation of raw electron micrographs (cter), picking/stack creation (window), reproducible 2-D classification (isac), reproducible initial model generation (viper), automatic gold-standard 3-D refinement (meridien), local resolution estimation and filtering (localres), and up to the 3-D sorting of different conformational states based on the statistical 3-D variability of the data (sort3D).

  • Primary Citation*

    T. Moriya, M. Saur, M. Stabrin, F. Merino, H. Voicu, Z. Huang, P. A. Penczek, S. Raunser, and C. Gatsogiannis. 2017. High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE. Journal of Visualized Experiments.


    • *Full citation information available through

  • Webinars

    Topic: SPHIRE: User-friendly pipeline for near-atomic resolution Cryo-EM structure determination with automatic validation mechanisms.
    Speaker: Toshio Moriya, Ph.D. Postdoctoral Fellow, Max Planck Institute of Molecular Physiology

    Host: Jason Key
    Recorded on: October 10, 2017

  • Keywords

    Electron Microscopy

  • Default Versions

    Linux 64:  20170602

  • Other Available Versions

      Linux 64:

      20161216
  • Developers

    Christos Gatsogiannis, Zhong Huang, Felipe Merino, Toshio Moriya, Pawel Penczek, Stefan Raunser, Michael Saur, Markus Stabrin.

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