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Harvard Cryo-EM Center
Harvard Medical School
Cryo-EM Specialist position at the Harvard Cryo-EM Center for Structural Biology
Introduction: Harvard Medical School, Boston Children's Hospital, Dana-Farber Cancer Institute, and Massachusetts General Hospital have joined to create a new cryo-EM center for structural biology in the Harvard community. The facility housing two FEI Titan Krios electron microscopes and one FEI Talos Arctica electron microscope, will open in the fall of 2018. We seek personnel who will train users, operate the microscopes and interface with a vibrant scientific user community in both single-particle and tomography projects. This position is ideal for scientists keen to use their skills to contribute to multiple projects from many research areas, and to take on science management responsibilities.
Job description. (1) Serve as the technical interface between the user and the microscopes: check microscope alignment, insert grids, set up user to record images. (2) Train users in cryo-grid preparation, in operation of software that runs the microscope and records the images. (3) Advise users on experimental strategy or (when appropriate) collaborate with them to assist in data recording, analysis, and structure determination. (4) Assist director in managing the facility.
This position will have a high degree of independence, opportunities to present at regional and national scientific symposia and workshops, and opportunities to develop collaborative relationships with Harvard investigators.
Requirements. (1) Ph.D. in a field related to structural biology such as cell biology or biochemistry. Experience in structural biology -- either x-ray crystallography or electron microscopy (or both) -- preferably at the postdoctoral level. Moderate or extensive EM experience preferred. Experience with cryo electron tomography is a plus. (2) Expertise in operating complex instrumentation and experience with sophisticated software systems. (3) Good communication and interpersonal skills. (4) Interest in becoming cryoEM expert and learning how to align and operate high-end microscopes at a very sophisticated level. (Potential career track as EM facility director, for example.)
To apply, please submit a cover letter and CV to firstname.lastname@example.org. In the Subject line of your email, please write: Cryo-EM Specialist.
Posting Date: October 29, 2018
The Deaconescu Lab at Brown University has two openings for postdoc candidates. The laboratory's interests lie primarily in stress responses, with particular emphasis on responses to DNA damage and the mechanochemistry of DNA-based motors. We utilize a combination of biochemical, biophysical and structural techniques. (e.g. X-ray crystallography, small-angle X-ray scattering, transmission electron microscopy). For examples of work, please see Vemu et al. Science (in press), Le TT et al. Cell (2018), Deaconescu et al. Photochemistry and Photobiology (2017), Kutter et al. JMB (2016), Szyk et al, Cell (2014), Deaconescu et al., PNAS (2012), Szyk et al., NSMB (2012) and Deaconescu et al., TIBS (2013).
A successful candidate should have an established track-record of publications in peer-reviewed journals; solid experience with protein biochemistry (including their purification from E.coli/yeast expression, functional characterization and assay development). Prior knowledge of crystallography and/or single-particle electron microscopy is highly desirable. Crystals/data at various stages of the structure determination process are available for immediate work. Must be highly motivated and work independently as well as in a team. Excellent spoken and written English are required. New Ph.D. graduates are encouraged to apply.
Interested candidates should send a CV, one page research experience summary and contact information for three references to email@example.com. Salary and starting date are negotiable. Please write Postdoctoral Candidate in the e-mail subject header. Brown University, an Ivy League school, is located less than one hour away by train from Boston. Lab webpage: https://deaconesculab.com
Posting Date: September 6, 2018
National Institutes of Health - NINDS & NHLBI
We are currently seeking an outstanding individual to join our team as a Senior Scientist in Cryo-Electron Microscopy at the National Institute of Neurological Disorders and Stroke in Bethesda. The successful candidate will join a multidisciplinary group with expertise in molecular biology, protein biochemistry, X-Ray crystallography and high-resolution fluorescence microscopy with a mission to provide molecular insights into important cytoskeletal regulators. The lab has regular access to a state-of-the art cryo-EM facility which includes a Titan Krios with a K2 direct electron detector with energy filter and will soon add a 200 kV Talos Arctica.
The Senior Scientist will:
Bring innovative structural biology technologies, with a focus on cryo-EM as a method for novel protein structure determination Design and conduct experiments for all aspects of structure determination by Cryo-EM, including grid preparation, data collection and processing and 3-D model building. Work closely with molecular biologists, protein scientists, biophysicists as part of an integrated structural biology effort Interface with the central trans-NIH cryo-EM facility for optimal data collection strategies Training, assisting and overseeing scientists in the group in sample preparation for cryo-EM, data collection and image processing Contribute to the strategic planning of our group in cryo-EM
A minimum of a Ph.D. or equivalent is required Postdoctoral experience in structural biology or related field is required A minimum of 3 years of research experience in single particle cryo-electron microscopy in industry or an academic institution is required Deep scientific expertise in structural biology and high-resolution structure determination is required Strong record of external publications and presentations is required Experience in scientific programming and ability to work with the latest relevant software packages Experience in cryo-electron tomography a plus Skills in working and communicating with scientists at all levels
Salary and benefits are generous and commensurate with research experience and accomplishments. The DC area is a great place to live with access to many cultural and outdoors activities. NINDS offers a rich and diverse scientific environment and the resources on campus are unparalleled. More information can be found at: https://neuroscience.nih.gov/ninds/rm_lab/ and https://neuroscience.nih.gov/ninds/Home.aspx
To apply: Please send a cover letter, curriculum vitae, bibliography, a one-page statement of research experience relevant to this position and the names of three references to Antonina@mail.nih.gov Applications will be considered until December 1, 2018.
Posting Date: August 24, 2018